Characterization of a 1980-swine recombinant influenza virus possessing H1 hemagglutinin and N2 neuraminidase similar to that of the earliest Hong Kong (H3N2) virus
Identifieur interne : 002470 ( Main/Exploration ); précédent : 002469; suivant : 002471Characterization of a 1980-swine recombinant influenza virus possessing H1 hemagglutinin and N2 neuraminidase similar to that of the earliest Hong Kong (H3N2) virus
Auteurs : K. Nerome ; Y. Yoshioka [Japon] ; S. Sakamoto ; H. Yasuhara [Japon] ; A. Oya [Japon]Source :
- Archives of Virology [ 0304-8608 ] ; 1985-09-01.
Descripteurs français
- KwdFr :
- ARN viral (analyse), Animaux, Humains, Hybridation d'acides nucléiques, Hémagglutination virale, Hémagglutinines virales (analyse), Oligonucléotides (analyse), Recombinaison génétique, Réplication virale, Sialidase (analyse), Sous-type H3N2 du virus de la grippe A, Suidae, Température, Virus de la grippe A (enzymologie), Virus de la grippe A (génétique), Virus de la grippe A (immunologie).
- MESH :
- analyse : ARN viral, Hémagglutinines virales, Oligonucléotides, Sialidase.
- enzymologie : Virus de la grippe A.
- génétique : Virus de la grippe A.
- immunologie : Virus de la grippe A.
- Animaux, Humains, Hybridation d'acides nucléiques, Hémagglutination virale, Recombinaison génétique, Réplication virale, Sous-type H3N2 du virus de la grippe A, Suidae, Température.
English descriptors
- KwdEn :
- Animals, Hemagglutination, Viral, Hemagglutinins, Viral (analysis), Humans, Influenza A Virus, H3N2 Subtype, Influenza A virus (enzymology), Influenza A virus (genetics), Influenza A virus (immunology), Neuraminidase (analysis), Nucleic Acid Hybridization, Oligonucleotides (analysis), RNA, Viral (analysis), Recombination, Genetic, Swine, Temperature, Virus Replication.
- MESH :
- chemical , analysis : Hemagglutinins, Viral, Neuraminidase, Oligonucleotides, RNA, Viral.
- enzymology : Influenza A virus.
- genetics : Influenza A virus.
- immunology : Influenza A virus.
- Teeft :
- Animals, Antigenic, Antigenic analysis, Antigenic characteristics, Antigenic determinants, Avian, Avian influenza, Avian influenza viruses, Avian population, Cent polyacrylamide, Different ages, Earliest hong kong, East asia, Edna segments, Erret serum, Experimental conditions, Further studies, Genetic reassortment, Growth characteristics, Growth comparison, Hemagglutination, Viral, Hemagglutinin, Hemagglutinin antigen, Hemagglutinin gene, High temperature, Hong kong, Human influenza, Human influenza viruses, Human population, Human viruses, Humans, Hybridization, Important reservoir, Influenza, Influenza A Virus, H3N2 Subtype, Influenza virus, Influenza virus neuraminidase, Influenza viruses, Late asian, Mdck cells, Molecular hybridization, Monoclonal, Monoclonal antibodies, Neuraminidase, Neuraminidase gene, Neutralizing antibodies, Nucleic Acid Hybridization, Oligonucleotide mapping, Oligonucleotide spots, Oligonueleotide, Oligonueleotide maps, Oligonueleotide spots, Open arrows, Open circles, Pandemic influenza, Parentm viruses, Plaque, Plaque formation, Possible origin, Present studies, Previous paper, Previous report, Recombinant, Recombinant virus, Recombinant viruses, Recombination, Genetic, Rna, Second dimension, Sequence homology, Swine, Swine influenza, Swine influenza virus, Swine population, Temperature, Virol, Virology, Virus, Virus Replication, Xylene cyanol.
Abstract
Summary: A recombinant (H1N2, formerly Hsw1N2), A/swine/Ehime/1/80 was found to possess antigenic, biological and genomic characteristics different from those of a previous A/swine/Kanagawa/2/78 (H1N2) strain (22). Five monoclonal antibodies to A/NJ/8/76 definitely differentiated the hemagglutinin molecules of the former virus from the latter, showing that these viruses differed, at least, at two antigenic determinants. Neuraminidase-inhibition tests with monoclonal antibodies to different H2N2 and H3N2 viruses revealed that the A/swine/Ehime/1/80 strain contained a neuraminidase very similar to that of the late human Asian (H2N2) and the earliest Hong Kong (H3N2) viruses. Growth comparison of swine and human isolates indicated that A/swine/Ehime/1/80 and A/swine/Shizuoka/1/78 (H1N1) failed to grow at 42°C, while A/swine/Kanagawa/2/78 and its possible parental virus, A/swine/Kanagawa/4/78 (H1N1) replicated efficiently at this stringent temperature. These results revealed that the viruses having growth characteristics similar to those of avian influenza virus were present in the swine population. RNA analysis by oligonucleotide mapping suggested that A/swine/Ehime/1/80 may be a recombinant between A/swine/Shizuoka/1/78-like and A/Aichi/2/68 (H3N2)-like viruses. To further determine the gene constellation of this recombinant virus, DNA-RNA hybridization was performed by using DNA segments complementary for swine (H1N1) virus RNA and the entire RNAs of three viruses. The molecular hybridization could define the genomic composition of the recombinant, indicating that only the neuraminidase gene of this virus is derived from the earliest Hong Kong (H3N2)-like virus and remaining seven genes from swine (H1N1) virus.
Url:
DOI: 10.1007/BF01309825
Affiliations:
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A</term><term>Suidae</term><term>Température</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Summary: A recombinant (H1N2, formerly Hsw1N2), A/swine/Ehime/1/80 was found to possess antigenic, biological and genomic characteristics different from those of a previous A/swine/Kanagawa/2/78 (H1N2) strain (22). Five monoclonal antibodies to A/NJ/8/76 definitely differentiated the hemagglutinin molecules of the former virus from the latter, showing that these viruses differed, at least, at two antigenic determinants. Neuraminidase-inhibition tests with monoclonal antibodies to different H2N2 and H3N2 viruses revealed that the A/swine/Ehime/1/80 strain contained a neuraminidase very similar to that of the late human Asian (H2N2) and the earliest Hong Kong (H3N2) viruses. Growth comparison of swine and human isolates indicated that A/swine/Ehime/1/80 and A/swine/Shizuoka/1/78 (H1N1) failed to grow at 42°C, while A/swine/Kanagawa/2/78 and its possible parental virus, A/swine/Kanagawa/4/78 (H1N1) replicated efficiently at this stringent temperature. These results revealed that the viruses having growth characteristics similar to those of avian influenza virus were present in the swine population. RNA analysis by oligonucleotide mapping suggested that A/swine/Ehime/1/80 may be a recombinant between A/swine/Shizuoka/1/78-like and A/Aichi/2/68 (H3N2)-like viruses. To further determine the gene constellation of this recombinant virus, DNA-RNA hybridization was performed by using DNA segments complementary for swine (H1N1) virus RNA and the entire RNAs of three viruses. The molecular hybridization could define the genomic composition of the recombinant, indicating that only the neuraminidase gene of this virus is derived from the earliest Hong Kong (H3N2)-like virus and remaining seven genes from swine (H1N1) virus.</div></front></TEI><affiliations><list><country><li>Japon</li></country><region><li>Région de Kantō</li></region><settlement><li>Tokyo</li></settlement></list><tree><noCountry><name sortKey="Nerome, K" sort="Nerome, K" uniqKey="Nerome K" first="K." last="Nerome">K. Nerome</name><name sortKey="Sakamoto, S" sort="Sakamoto, S" uniqKey="Sakamoto S" first="S." last="Sakamoto">S. Sakamoto</name></noCountry><country name="Japon"><region name="Région de Kantō"><name sortKey="Yoshioka, Y" sort="Yoshioka, Y" uniqKey="Yoshioka Y" first="Y." last="Yoshioka">Y. Yoshioka</name></region><name sortKey="Oya, A" sort="Oya, A" uniqKey="Oya A" first="A." last="Oya">A. Oya</name><name sortKey="Yasuhara, H" sort="Yasuhara, H" uniqKey="Yasuhara H" first="H." last="Yasuhara">H. Yasuhara</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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